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Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – <t>galvanometric</t> scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.
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Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – <t>galvanometric</t> scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.
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galvanometric scanning mirrors  (Thorlabs)
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Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – <t>galvanometric</t> scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.
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lens-based galvanometric mirror scan/descan unit 2× cls-sl and gvs201  (Thorlabs)
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Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – <t>galvanometric</t> scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.
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Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – galvanometric scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.

Journal: Biomedical Optics Express

Article Title: Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils

doi: 10.1364/BOE.504304

Figure Lengend Snippet: Schematic diagram of the microscope setup (a) and coordinate system used here (b). The following abbreviations are used: L – laser, SM – galvanometric scan mirrors, PSG – polarization state generator, LC – liquid crystal variable retarder, EO – excitation objective, CO – collection objective, PSA – polarization state analyzer, PM – optical power meter, F – filter, PMT – photomultiplier tube. The dashed arrows in (a) indicate components that can be added for calibration and CD-SHG imaging (see below). The fibril is shown in orange in (b) and the imaging plane is shaded in gray.

Article Snippet: Briefly, the laser beam was raster scanned across the sample using a pair of galvanometric scan mirrors (ScannerMAX, Pangolin Laser Systems, Inc.) with a pixel dwell time of 6 μs, to create an image with a pixel size of 180 nm and size 18 × 18 or 36 × 36 μm.

Techniques: Microscopy, Imaging